Transient binding events are a challenging issue in enzymology. Here we demostrate a time-based ITC approach to human flavincontaining monooxygenase 3, an important drug metabolising enzyme. We measure kinetic constants and we demonstrate how this approach can be exploited for measuring the inhibiton of the conversion of the key substrate trimethylamine into trimethylamine N-oxide.
A direct time-based ITC approach for substrate turnover measurements demonstrated on human FMO3
Catucci, Gianluca
First
;Sadeghi, Sheila J.;Gilardi, Gianfranco
Last
2019-01-01
Abstract
Transient binding events are a challenging issue in enzymology. Here we demostrate a time-based ITC approach to human flavincontaining monooxygenase 3, an important drug metabolising enzyme. We measure kinetic constants and we demonstrate how this approach can be exploited for measuring the inhibiton of the conversion of the key substrate trimethylamine into trimethylamine N-oxide.
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2019-Catucci-ITC.pdf
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