NAD+ plays central roles in a wide array of normal and pathological conditions. Inhibition of NAD+ biosynthesis can be exploited therapeutically in cancer, including melanoma. To obtain quantitation of NAD+ levels in live cells and to address the issue of the compartmentalization of NAD+ biosynthesis, we exploited a recently described, genetically-encoded NAD+ biosensor (LigA-cpVENUS), which was targeted to the cytosol, mitochondria and nuclei of BRAF-V600E A375 melanoma cells, a model of metastatic melanoma (MM).

Subcellular characterization of NAD+ biosynthesis in metastatic melanoma by using organelle-specific biosensors

Gaudino, Federica;Managò, Antonella;Audrito, Valentina;Vaisitti, Tiziana;Deaglio, Silvia
Last
2019

Abstract

NAD+ plays central roles in a wide array of normal and pathological conditions. Inhibition of NAD+ biosynthesis can be exploited therapeutically in cancer, including melanoma. To obtain quantitation of NAD+ levels in live cells and to address the issue of the compartmentalization of NAD+ biosynthesis, we exploited a recently described, genetically-encoded NAD+ biosensor (LigA-cpVENUS), which was targeted to the cytosol, mitochondria and nuclei of BRAF-V600E A375 melanoma cells, a model of metastatic melanoma (MM).
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Gaudino, Federica; Manfredonia, Ilaria; Managò, Antonella; Audrito, Valentina; Raffaelli, Nadia; Vaisitti, Tiziana; Deaglio, Silvia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1711580
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