The employment of ionizing radiation is a powerful tool in cancer therapy, but beyond targeted effects, many studies have highlighted the relevance of its off-target consequences. An exhaustive understanding of the mechanisms underlying these effects is still missing, and no real-time data about signals released by cells during irradiation are presently available. We employed a synchrotron X-ray nanobeam to perform the first real-time simultaneous measurement of both X-ray irradiation and in vitro neurotransmitter release from individual adrenal phaeochromocytoma (PC12) cells plated over a diamond-based multielectrode array. We have demonstrated that, in specific conditions, X-rays can alter cell activity by promoting dopamine exocytosis, and such an effect is potentially very attractive for a more effective treatment of tumors.

Triggering Neurotransmitters Secretion from Single Cells by X-ray Nanobeam Irradiation

F. Picollo
;
G. Tomagra;V. Bonino;V. Carabelli;L. Mino;P. Olivero;M. Truccato
2020

Abstract

The employment of ionizing radiation is a powerful tool in cancer therapy, but beyond targeted effects, many studies have highlighted the relevance of its off-target consequences. An exhaustive understanding of the mechanisms underlying these effects is still missing, and no real-time data about signals released by cells during irradiation are presently available. We employed a synchrotron X-ray nanobeam to perform the first real-time simultaneous measurement of both X-ray irradiation and in vitro neurotransmitter release from individual adrenal phaeochromocytoma (PC12) cells plated over a diamond-based multielectrode array. We have demonstrated that, in specific conditions, X-rays can alter cell activity by promoting dopamine exocytosis, and such an effect is potentially very attractive for a more effective treatment of tumors.
20
5
3889
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X-ray synchrotron nanoirradiation dopamine exocytosis diamond microelectrode arrays photocurrent detection radiobiology
F. Picollo, G. Tomagra, V. Bonino, V. Carabelli, L. Mino, P. Olivero, A. Pasquarelli, M. Truccato
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1738678
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