RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3′ untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.

Genome-scale deconvolution of RNA structure ensembles

Edoardo Morandi
First
;
Francesca Anselmi;Salvatore Oliviero
Co-last
;
Danny Incarnato
Co-last
2021

Abstract

RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3′ untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.
18
249
252
https://www.nature.com/articles/s41592-021-01075-w
RNA, RNA structure, SARS-COV-2
Edoardo Morandi, Ilaria Manfredonia, Lisa M. Simon, Francesca Anselmi, Martijn J. van Hemert, Salvatore Oliviero, Danny Incarnato,
File in questo prodotto:
File Dimensione Formato  
Morandi et al.pdf

Accesso aperto con embargo fino al 22/08/2021

Descrizione: versione finale degli autori
Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 213.26 kB
Formato Adobe PDF
213.26 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1771193
Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 20
  • ???jsp.display-item.citation.isi??? 16
social impact