We prospectively compared the performance of RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays directly from blood cultures spiked with 130 characterized Enterobacterales isolates. Overall, both assays yielded 100% sensitivity to detect KPC-type carbapenemases and OXA-48-like carbapenemases. Both assays failed to detect KPC-31 and KPC-33, D179Y point mutation variants of KPC-3 and KPC-2, that are deprived of carbapenemase activity and confer resistance to ceftazidime-avibactam. On blood culture bacterial pellets, NDM- and VIM-type carbapenemases were detected in 50.0% and 52.2%, respectively, by RESIST-5 O.O.K.N.V. vs 100% by NG-Test Carba 5. The sensitivity of RESIST-5 O.O.K.N.V. improved to 100% and 95.6%, respectively, by performing the assay on 4-h early subculture.

RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays for the rapid detection of carbapenemase-producing Enterobacterales from positive blood cultures: a comparative study

Bianco G.;Boattini M.;Iannaccone M.;Zanotto E.;Zaccaria T.;Cavallo R.;Costa C.
2020

Abstract

We prospectively compared the performance of RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays directly from blood cultures spiked with 130 characterized Enterobacterales isolates. Overall, both assays yielded 100% sensitivity to detect KPC-type carbapenemases and OXA-48-like carbapenemases. Both assays failed to detect KPC-31 and KPC-33, D179Y point mutation variants of KPC-3 and KPC-2, that are deprived of carbapenemase activity and confer resistance to ceftazidime-avibactam. On blood culture bacterial pellets, NDM- and VIM-type carbapenemases were detected in 50.0% and 52.2%, respectively, by RESIST-5 O.O.K.N.V. vs 100% by NG-Test Carba 5. The sensitivity of RESIST-5 O.O.K.N.V. improved to 100% and 95.6%, respectively, by performing the assay on 4-h early subculture.
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Blood culture; Carbapenemase detection; Ceftazidime-avibactam resistance; D179Y; Enterobacterales; Immunochromatographic assay; Anti-Bacterial Agents; Azabicyclo Compounds; Bacterial Proteins; Blood Culture; Ceftazidime; Drug Combinations; Enterobacteriaceae; Enterobacteriaceae Infections; Humans; Immunoassay; Microbial Sensitivity Tests; Prospective Studies; Reagent Kits, Diagnostic; Sensitivity and Specificity; beta-Lactamases
Bianco G.; Boattini M.; van Asten S.A.V.; Iannaccone M.; Zanotto E.; Zaccaria T.; Bernards A.T.; Cavallo R.; Costa C.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1774659
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