Molecular cloning is the collection of experimental procedures required to isolate and expand a specific fragment of DNA into a host organism in order to create a large number of identical copies. On top of allowing the study of a single DNA sequence of interest, molecular cloning is a powerful technique that permits the generation of complex combinations of DNA fragments for the most disparate downstream applications. As such, this process is key to most modern biomedical basic research studies and translational applications. This chapter will present an overview of modern molecular cloning procedures and their potential applications, and provide the basic concepts and protocols required to perform a traditional cloning experiment based on restriction enzyme digestion and ligation of two DNA fragments.
Methods of Cloning
Bertero A.First
;
2017-01-01
Abstract
Molecular cloning is the collection of experimental procedures required to isolate and expand a specific fragment of DNA into a host organism in order to create a large number of identical copies. On top of allowing the study of a single DNA sequence of interest, molecular cloning is a powerful technique that permits the generation of complex combinations of DNA fragments for the most disparate downstream applications. As such, this process is key to most modern biomedical basic research studies and translational applications. This chapter will present an overview of modern molecular cloning procedures and their potential applications, and provide the basic concepts and protocols required to perform a traditional cloning experiment based on restriction enzyme digestion and ligation of two DNA fragments.
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