The binding of strigolactones to their receptor, the α/β hydrolase DWARF14 (D14), leads to the modulation of transcriptional activity by destabilization of specific transcriptional corepressors via proteasomal degradation. Subsequently, strigolactones also promote D14 degradation by the same pathway. Here we describe an innovative quantitative bioassay based on Arabidopsis transgenic lines expressing AtD14 fused to the firefly luciferase, developed to identify new strigolactone analogs capable to activate the strigolactone signaling.

Evaluation of the Bioactivity of Strigolactone-Related Molecules by a Quantitative Luminometer Bioassay

Francesca Cardinale;Ivan Visentin
Last
2021-01-01

Abstract

The binding of strigolactones to their receptor, the α/β hydrolase DWARF14 (D14), leads to the modulation of transcriptional activity by destabilization of specific transcriptional corepressors via proteasomal degradation. Subsequently, strigolactones also promote D14 degradation by the same pathway. Here we describe an innovative quantitative bioassay based on Arabidopsis transgenic lines expressing AtD14 fused to the firefly luciferase, developed to identify new strigolactone analogs capable to activate the strigolactone signaling.
2021
Strigolactones. Methods and Protocols
Humana
Methods in Molecular Biology
2309
191
200
978-1-0716-1428-0
978-1-0716-1429-7
Bioassay, Luciferase, Perception, Plant hormones, Strigolactones, DWARF14
Elena Sánchez; Pilar Cubas; Francesca Cardinale; Ivan Visentin
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1810940
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