Objective: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. Methods and Results: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. Conclusion: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. © 2010 American Heart Association, Inc.

Role of delta-like-4/notch in the formation and wiring of the lymphatic network in zebrafish

Mazzone M.;Bruyere F.;
2010-01-01

Abstract

Objective: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development. Methods and Results: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates. Conclusion: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network. © 2010 American Heart Association, Inc.
2010
30
9
1695
1702
gene silencing; lymphangiogenesis; notch signaling; vascular biology; zebrafish; Animals; Animals, Genetically Modified; Biomarkers; COS Cells; Cell Movement; Cell Proliferation; Chlorocebus aethiops; Coculture Techniques; Embryo, Nonmammalian; Endothelial Cells; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Gene Silencing; Humans; Intracellular Signaling Peptides and Proteins; Luminescent Proteins; Lymphatic System; Membrane Proteins; RNA, Messenger; Receptors, Notch; Thoracic Duct; Zebrafish; Zebrafish Proteins; Lymphangiogenesis; Signal Transduction
Geudens I.; Herpers R.; Hermans K.; Segura I.; Ruiz De Almodovar C.; Bussmann J.; De Smet F.; Vandevelde W.; Hogan B.M.; Siekmann A.; Claes F.; Moore ...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1841158
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