ALK rearrangement is the only druggable oncogenic driver detectable by immunohistochemistry (IHC) not requiring further confirmation of positivity in accessing first-line specific inhibitors. ALK-positive patients experience clinical benefit from pemetrexed-based chemotherapy possibly due to lower thymidylate synthase (TS) levels. This study assesses agreement with three different ALK IHC clones in 37 FISH-positive NSCLC. TS expression by real time (RT)-PCR was compared with ALK FISH-negative cases.

Immunohistochemistry with 3 different clones in anaplastic lymphoma kinase fluorescence in situ hybridization positive non-small-cell lung cancer with thymidylate synthase expression analysis: a multicentre, retrospective, Italian study

Bironzo, Paolo;Monica, Valentina;Righi, Luisella;Novello, Silvia
2022-01-01

Abstract

ALK rearrangement is the only druggable oncogenic driver detectable by immunohistochemistry (IHC) not requiring further confirmation of positivity in accessing first-line specific inhibitors. ALK-positive patients experience clinical benefit from pemetrexed-based chemotherapy possibly due to lower thymidylate synthase (TS) levels. This study assesses agreement with three different ALK IHC clones in 37 FISH-positive NSCLC. TS expression by real time (RT)-PCR was compared with ALK FISH-negative cases.
2022
Sep 9
1
10
ALK; FISH; immunohistochemistry; non-small-cell lung cancer; thymidylate synthase
Bironzo, Paolo; Melocchi, Laura; Monica, Valentina; Trebeschi, Dario; Barbieri, Fausto; Maiello, Evaristo; Migliorino, Maria Rita; Lombardi, Alessandra; Tiseo, Marcello; Righi, Luisella; Graziano, Paolo; Rossi, Giulio; Novello, Silvia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1873921
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