The investigation of secondary effects induced by ionizing radiation represents a new and ever-growing research field in radiobiology. This new paradigm cannot be investigated only using standard instrumentation and methodologies, but rather requires novel technologies to achieve significant progress. In this framework, we developed diamond-based sensors that allow simultaneous real-time measurements with a high spatial resolution of the secretory activity of a network of cells cultured on the device, as well as of the dose at which they are exposed during irradiation experiments. The devices were functionally characterized by testing both the above- mentioned detection schemes, namely: amperometric measurements of neurotransmitter release from excitable cells (such as dopamine or adrenaline) and dosimetric evaluation using different ionizing particles (alpha particle and X-ray photons). Finally, the sensors were employed to investigate the effects induced by X-rays on the exocytotic activity of PC12 neuroendocrine cells by monitoring the modulation of the dopamine release in real- time.

Diamond-based sensors for in vitro cellular radiobiology: Simultaneous detection of cell exocytic activity and ionizing radiation

Tomagra, Giulia;Peroni, Giulia;Aprà, Pietro;Bonino, Valentina;Campostrini, Matteo;Carabelli, Valentina;Collà Ruvolo, Cecilia;Lo Giudice, Alessandro;Guidorzi, Laura;Mino, Lorenzo;Olivero, Paolo;Pacher, Luca;Picariello, Fabio;Re, Alessandro;Truccato, Marco;Varzi, Veronica;Vittone, Ettore;Picollo, Federico
2023-01-01

Abstract

The investigation of secondary effects induced by ionizing radiation represents a new and ever-growing research field in radiobiology. This new paradigm cannot be investigated only using standard instrumentation and methodologies, but rather requires novel technologies to achieve significant progress. In this framework, we developed diamond-based sensors that allow simultaneous real-time measurements with a high spatial resolution of the secretory activity of a network of cells cultured on the device, as well as of the dose at which they are exposed during irradiation experiments. The devices were functionally characterized by testing both the above- mentioned detection schemes, namely: amperometric measurements of neurotransmitter release from excitable cells (such as dopamine or adrenaline) and dosimetric evaluation using different ionizing particles (alpha particle and X-ray photons). Finally, the sensors were employed to investigate the effects induced by X-rays on the exocytotic activity of PC12 neuroendocrine cells by monitoring the modulation of the dopamine release in real- time.
2023
220
114876
114884
Diamond sensor Ion beam lithography Ionizing radiation detection Dopamine exocytosis Radiobiology
Tomagra, Giulia; Peroni, Giulia; Aprà, Pietro; Bonino, Valentina; Campostrini, Matteo; Carabelli, Valentina; Collà Ruvolo, Cecilia; Lo Giudice, Alessandro; Guidorzi, Laura; Mino, Lorenzo; Olivero, Paolo; Pacher, Luca; Picariello, Fabio; Re, Alessandro; Rigato, Valentino; Truccato, Marco; Varzi, Veronica; Vittone, Ettore; Picollo, Federico
File in questo prodotto:
File Dimensione Formato  
2023_Tomagra_Diamond-based sensors for in vitro cellular radiobiology Simultaneous detection of cell exocytic activity and ionizing radiation.pdf

Accesso aperto

Tipo di file: PDF EDITORIALE
Dimensione 3.79 MB
Formato Adobe PDF
3.79 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1879634
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 0
social impact