Terpenes are natural molecules of valuable interest for different industrial applications. Cytochromes P450 enzymes can functionalize terpenoids to form high value oxidized derivatives in a green and sustainable manner, representing a valid alternative to chemical catalysis. In this work, an enhanced and specific epoxidation activity of cytochrome P450 BM3 mutants was found for the terpenes geraniol and linalool. This is the first report showing the epoxidation of linalool by P450 BM3 and its mutant A2 (Asp251Gly/Gln307His) with the formation of valuable oxide derivatives, highlighting the relevance of this enzymes for industrial applications.

Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives

Correddu, Danilo
First
;
Di Nardo, Giovanna;Catucci, Gianluca;Prandi, Cristina;Blangetti, Marco;Bellomo, Chiara;Bonometti, Elisabetta;Viscardi, Guido;Gilardi, Gianfranco
Last
2022-01-01

Abstract

Terpenes are natural molecules of valuable interest for different industrial applications. Cytochromes P450 enzymes can functionalize terpenoids to form high value oxidized derivatives in a green and sustainable manner, representing a valid alternative to chemical catalysis. In this work, an enhanced and specific epoxidation activity of cytochrome P450 BM3 mutants was found for the terpenes geraniol and linalool. This is the first report showing the epoxidation of linalool by P450 BM3 and its mutant A2 (Asp251Gly/Gln307His) with the formation of valuable oxide derivatives, highlighting the relevance of this enzymes for industrial applications.
2022
12
52
33964
33969
https://pubs.rsc.org/en/content/articlelanding/2022/ra/d2ra06029a
Correddu, Danilo; Helmy Aly, Sabrina; Di Nardo, Giovanna; Catucci, Gianluca; Prandi, Cristina; Blangetti, Marco; Bellomo, Chiara; Bonometti, Elisabetta; Viscardi, Guido; Gilardi, Gianfranco
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1884209
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