The translation of the genetic code into protein products is characterized by the identification of triplets called codons that allow the incorporation of an amino acid. The heterologous expression of human genes in bacterial cells can encounter translational problems due to the presence of codons that are normally used with low frequency in bacteria. In Escherichia coli the codons AGG and AGA are the less frequent and are known to cause translational problems, particularly when they are present in tandem. Consecutive AGG-AGA can lead to a premature stop in a sequence dependent manner causing the production of a truncated protein.
Issue Highlights
Correddu D.First
;
2020-01-01
Abstract
The translation of the genetic code into protein products is characterized by the identification of triplets called codons that allow the incorporation of an amino acid. The heterologous expression of human genes in bacterial cells can encounter translational problems due to the presence of codons that are normally used with low frequency in bacteria. In Escherichia coli the codons AGG and AGA are the less frequent and are known to cause translational problems, particularly when they are present in tandem. Consecutive AGG-AGA can lead to a premature stop in a sequence dependent manner causing the production of a truncated protein.
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