Molecular assays may constitute a valid method for timely prediction of antimicrobial resistance and optimization of empirical antibiotic therapies. This study assessed ELITe MGB assays of blood cultures to detect the main carbapenemase and extended-spectrum beta-lactamase (ESBL) genes, Staphylococcus aureus and mec genes in less than 3 h. Excellent agreement was found between the results of genotypic and conventional phenotypic approaches. Retrospective analysis of medical records revealed that approximately 50% of bloodstream infections caused by ESBL-producing Enterobacteriaceae, carbapenemase-producing Enterobacteriaceae or meticillin-resistant S. aureus were initially treated with inactive drugs. Overall, 36.3% of patients could have been treated with appropriate therapy at least 24 h earlier if molecular data had been used.

Detection of antibiotic resistance genes from blood cultures: performance assessment and potential impact on antibiotic therapy management

Bianco Gabriele
First
;
Boattini Matteo;Iannaccone Marco;Sidoti Francesca;Cavallo Rossana;Costa Cristina
Last
2019-01-01

Abstract

Molecular assays may constitute a valid method for timely prediction of antimicrobial resistance and optimization of empirical antibiotic therapies. This study assessed ELITe MGB assays of blood cultures to detect the main carbapenemase and extended-spectrum beta-lactamase (ESBL) genes, Staphylococcus aureus and mec genes in less than 3 h. Excellent agreement was found between the results of genotypic and conventional phenotypic approaches. Retrospective analysis of medical records revealed that approximately 50% of bloodstream infections caused by ESBL-producing Enterobacteriaceae, carbapenemase-producing Enterobacteriaceae or meticillin-resistant S. aureus were initially treated with inactive drugs. Overall, 36.3% of patients could have been treated with appropriate therapy at least 24 h earlier if molecular data had been used.
2019
102
4
465
469
Antimicrobial susceptibility ; Molecular testing; Blood culture; Antimicrobial stewardship; Multi-drug resistance
Bianco Gabriele; Boattini Matteo; Iannaccone Marco; Sidoti Francesca; Cavallo Rossana; Costa Cristina
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1901445
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