Cultures enriched in glioblastoma stem-like cells (GSCs) are prominent in vitro models to investigate molecular determinants and therapeutic targets of glioblastoma; however, conventional GSC derivation protocols fail to preserve GSC heterogeneity. Here, we present a protocol for the propagation of heterogeneous GSC cultures starting from cell resuspensions containing the entire tumor mass. We describe steps for isolation of GSCs and their maintenance and expansion in culture. We then detail procedures for preliminary analysis to be performed on freshly isolated material. For complete details on the use and execution of this protocol, please refer to De Bacco et al.1.

Protocol for in vitro establishment of heterogeneous stem-like cultures derived from whole human glioblastoma tumors

De Bacco, Francesca
First
;
Orzan, Francesca
;
Prelli, Marta;Boccaccio, Carla
Last
2023-01-01

Abstract

Cultures enriched in glioblastoma stem-like cells (GSCs) are prominent in vitro models to investigate molecular determinants and therapeutic targets of glioblastoma; however, conventional GSC derivation protocols fail to preserve GSC heterogeneity. Here, we present a protocol for the propagation of heterogeneous GSC cultures starting from cell resuspensions containing the entire tumor mass. We describe steps for isolation of GSCs and their maintenance and expansion in culture. We then detail procedures for preliminary analysis to be performed on freshly isolated material. For complete details on the use and execution of this protocol, please refer to De Bacco et al.1.
2023
4
4
102705
102723
Cancer; Cell culture; Cell isolation; Flow Cytometry; Stem Cells
De Bacco, Francesca; Orzan, Francesca; Casanova, Elena; Prelli, Marta; Boccaccio, Carla
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1946411
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