Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing. © 2012 American Chemical Society.

Direct imaging of DNA fibers: The visage of double helix

Limongi T.;
2012-01-01

Abstract

Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing. © 2012 American Chemical Society.
2012
12
12
6453
6458
DNA bundles direct imaging; molecular dynamics simulations; nanofabricated micropillars; superhydrophobic surface; transmission electron microscopy
Gentile F.; Moretti M.; Limongi T.; Falqui A.; Bertoni G.; Scarpellini A.; Santoriello S.; Maragliano L.; Proietti Zaccaria R.; Di Fabrizio E.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1955518
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