Objectives: Surviving Sepsis Campaign (SSC) defined Sepsis as "life-threatening organ dysfunction caused by a dysregulated host response to infection" (De Backer D et al, Crit Care Med, n.d.). Sepsis remains one of the leading causes of morbidity and mortality (17-65% (De Oliveira DC, Arq Bras Cardiol Sociedade Brasileira de Cardiologia - SBC 94:352-6, 2010)) worldwide and it still remains a challenge to be defined and for which an appropriate treatment is desired (Chiu and Legrand, Curr Opin Anaesthesiol 34:71-6, 2021). Different studies have been conducted on genes coding for inflammatory cytokines whose could predispose to the development of sepsis [e.g., IL-10 PD1 and WT1] (Gupta DL et al, Infectious Process Sepsis, 202). Design: This multicentric observational prospective study aims to evaluate blinding the genetic expression kinetics of different molecules involved in the inflammatory process, IL10, PD1 and WT1, to search for a possible molecular predictive marker of sepsis. Setting: Nine University teaching Hospitals in Italy take part in this study in collaboration with the Department of Applied Science (DISBA) of the University of Basilicata. Participants: One hundred sixty-two patients, under elective cardiac and on pump surgery were enrolled in the study. Interventions: From each patient 4 blood samples were collected during and at the end of the surgery, following the study design. Measurements and main results: We observed, 30 min after the start of the surgery, lower gene expression levels of IL10 and PD1 in septic patients compared to non-septic (p < 0.05), but considering all the timepoint there are differences in gene expression modulation between the groups. Conclusion: These results confirmed the dysregulated immune response in septic patients compared to non-septic, highlight how a measurement of the gene expression could help to optimize procedures and pay attention to more susceptible patients.
Gene expression kinetics in Sepsis After Cardiac Surgery (SACS): a multicentric prospective observational study
Beccaria, Stefano;Costamagna, Andrea;Brazzi, Luca;
2025-01-01
Abstract
Objectives: Surviving Sepsis Campaign (SSC) defined Sepsis as "life-threatening organ dysfunction caused by a dysregulated host response to infection" (De Backer D et al, Crit Care Med, n.d.). Sepsis remains one of the leading causes of morbidity and mortality (17-65% (De Oliveira DC, Arq Bras Cardiol Sociedade Brasileira de Cardiologia - SBC 94:352-6, 2010)) worldwide and it still remains a challenge to be defined and for which an appropriate treatment is desired (Chiu and Legrand, Curr Opin Anaesthesiol 34:71-6, 2021). Different studies have been conducted on genes coding for inflammatory cytokines whose could predispose to the development of sepsis [e.g., IL-10 PD1 and WT1] (Gupta DL et al, Infectious Process Sepsis, 202). Design: This multicentric observational prospective study aims to evaluate blinding the genetic expression kinetics of different molecules involved in the inflammatory process, IL10, PD1 and WT1, to search for a possible molecular predictive marker of sepsis. Setting: Nine University teaching Hospitals in Italy take part in this study in collaboration with the Department of Applied Science (DISBA) of the University of Basilicata. Participants: One hundred sixty-two patients, under elective cardiac and on pump surgery were enrolled in the study. Interventions: From each patient 4 blood samples were collected during and at the end of the surgery, following the study design. Measurements and main results: We observed, 30 min after the start of the surgery, lower gene expression levels of IL10 and PD1 in septic patients compared to non-septic (p < 0.05), but considering all the timepoint there are differences in gene expression modulation between the groups. Conclusion: These results confirmed the dysregulated immune response in septic patients compared to non-septic, highlight how a measurement of the gene expression could help to optimize procedures and pay attention to more susceptible patients.
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