This research embarked on the creation of a multi-parent advanced generation inter-cross (MAGIC) population specifically designed for pepper (Capsicum annuum L.), featuring eight founder accessions, each distinguished by their unique phenotypic attributes. To begin, we conducted comprehensive whole-genome resequencing (~27x) of these parental lines, uncovering approximately 29.6 million polymorphisms, comprising 95% SNPs and 5% InDels, along with the identification of 1.2 million Structural Variants (SV). Simultaneously, we phenotyped the eight founders using 22 agronomic descriptors, including their resistance to key viral pathogens such as Tomato Spotted wilt virus (TSWV), Potato virus Y (PVY) P0, Tobacco mosaic virus (TMV), Pepper mild mottle virus (PMMV) P1.2, and P1.2.3. The comprehensive characterization of both the morpho-agronomic and genomic aspects of the MAGIC parents serve as a key foundation for dissecting the genetic diversity within our population. Moreover, the invaluable resources generated by our study aim to empower the broader pepper research community, thereby enhancing genetic and genomic investigations and providing crucial support for targeted breeding initiatives aimed at elevating the quality and productivity of pepper cultivars.
MAGIC in Capsicum annuum: bridging phenotypic and genomic dimensions
Martina, M.;Barchi, L.;Acquadro, A.
;Portis, E.
2025-01-01
Abstract
This research embarked on the creation of a multi-parent advanced generation inter-cross (MAGIC) population specifically designed for pepper (Capsicum annuum L.), featuring eight founder accessions, each distinguished by their unique phenotypic attributes. To begin, we conducted comprehensive whole-genome resequencing (~27x) of these parental lines, uncovering approximately 29.6 million polymorphisms, comprising 95% SNPs and 5% InDels, along with the identification of 1.2 million Structural Variants (SV). Simultaneously, we phenotyped the eight founders using 22 agronomic descriptors, including their resistance to key viral pathogens such as Tomato Spotted wilt virus (TSWV), Potato virus Y (PVY) P0, Tobacco mosaic virus (TMV), Pepper mild mottle virus (PMMV) P1.2, and P1.2.3. The comprehensive characterization of both the morpho-agronomic and genomic aspects of the MAGIC parents serve as a key foundation for dissecting the genetic diversity within our population. Moreover, the invaluable resources generated by our study aim to empower the broader pepper research community, thereby enhancing genetic and genomic investigations and providing crucial support for targeted breeding initiatives aimed at elevating the quality and productivity of pepper cultivars.
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