This study aimed to compare the sealing ability of different calcium silicate-based and epoxy-resin endodontic sealers with single-cone cold technique through confocal laser scanning microscope (CLSM) and micro computed tomography (MicroCT) analysis. An in-vitro evaluation was conducted of voids and gaps occurring within the sealer and at its interfaces with the cone and canal walls, as well as of the penetration depth of the materials examined. Sixty-five single-rooted teeth with fully formed apex were collected. After root canal shaping the specimens were distributed in six different groups (n = 10) and a control group (n = 5): Group AH Plus Epoxy Resin Sealer (Dentsply Sirona), Group AH Plus Bioceramic Sealer (Dentsply Sirona), Group BC Sealer (Brasseler), Group BC Sealer HiFlow (Brasseler), Group BioRoot Flow Sealer (Septodont), group experimental sealer (Dentsply Sirona). All canals were sealed with single cone technique with the addition of a fluorescent dye. The specimens were MicroCT scanned and sectioned at four different levels of analysis for the CLSM evaluation. The differences between groups were analyzed with one-way ANOVA and post-hoc Bonferroni test (P < 0.05). Kruskall–Wallis and Mann–Whitney U tests were used for the non-parametric statistical analysis (P < 0.05). The microCT analysis reported an higher presence of voids and empty spaces for BioRoot Flow Sealer (Septodont) (P < 0.001). The CLMS imaging showed no statistical difference between the groups at the different points of analysis except for AH Plus Epoxy Resin Sealer (Dentsply Sirona) that showed a higher rate of empty spaces at the apical level (P = 0.044). No statistical differences were recorded for the total percentage of CLSM gap areas between groups. Interestingly, voids were most prevalent in the coronal and middle sections of all samples, corresponding to regions, where the canal is typically more oval (P = 0.03). Among the materials tested, the Experimental Sealer (Dentsply Sirona) achieved the deepest penetration into dentinal tubules (P = 0.038). The sealing performance of endodontic sealers used with the single-cone cold technique appears adequate and depends on both the properties of the sealer and the anatomy of the root canal.
Confocal laser scanning and microCT evaluation of the intratubular penetration and sealing ability of calcium silicate and epoxy-resin endodontic sealers
Alovisi, Mario;Fenoglio, Vittorio;Berutti, Elio;Comba, Allegra;Pasqualini, Damiano
2026-01-01
Abstract
This study aimed to compare the sealing ability of different calcium silicate-based and epoxy-resin endodontic sealers with single-cone cold technique through confocal laser scanning microscope (CLSM) and micro computed tomography (MicroCT) analysis. An in-vitro evaluation was conducted of voids and gaps occurring within the sealer and at its interfaces with the cone and canal walls, as well as of the penetration depth of the materials examined. Sixty-five single-rooted teeth with fully formed apex were collected. After root canal shaping the specimens were distributed in six different groups (n = 10) and a control group (n = 5): Group AH Plus Epoxy Resin Sealer (Dentsply Sirona), Group AH Plus Bioceramic Sealer (Dentsply Sirona), Group BC Sealer (Brasseler), Group BC Sealer HiFlow (Brasseler), Group BioRoot Flow Sealer (Septodont), group experimental sealer (Dentsply Sirona). All canals were sealed with single cone technique with the addition of a fluorescent dye. The specimens were MicroCT scanned and sectioned at four different levels of analysis for the CLSM evaluation. The differences between groups were analyzed with one-way ANOVA and post-hoc Bonferroni test (P < 0.05). Kruskall–Wallis and Mann–Whitney U tests were used for the non-parametric statistical analysis (P < 0.05). The microCT analysis reported an higher presence of voids and empty spaces for BioRoot Flow Sealer (Septodont) (P < 0.001). The CLMS imaging showed no statistical difference between the groups at the different points of analysis except for AH Plus Epoxy Resin Sealer (Dentsply Sirona) that showed a higher rate of empty spaces at the apical level (P = 0.044). No statistical differences were recorded for the total percentage of CLSM gap areas between groups. Interestingly, voids were most prevalent in the coronal and middle sections of all samples, corresponding to regions, where the canal is typically more oval (P = 0.03). Among the materials tested, the Experimental Sealer (Dentsply Sirona) achieved the deepest penetration into dentinal tubules (P = 0.038). The sealing performance of endodontic sealers used with the single-cone cold technique appears adequate and depends on both the properties of the sealer and the anatomy of the root canal.
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