A method is presented for the separation and detection of eight synthetic corticosteroids in bovine liver tissues. Following sample preparation, the samples were separated by isocratic LC (mobile phase: 85% methanol, 15% dichloromethane) using a porous graphite (PGC) column and detected by ion trap MS-MS, using an atmospheric pressure chemical ionization (APCI) source and monitoring positive ions. An octaclecyl-silica phase system was also used for comparison. With the PGC stationary phase the critical isomer separation of dexamethasone and betamethasone was achieved. The performances of MS-MS, MS3, selected ion monitoring (SIM) and selected reaction monitoring (SRM) detection modes were checked and best conditions of sensitivity were obtained in MS-MS mode.
Separation and determination of synthetic corticosteroids in bovine liver by LC-ion-trap-MS-MS on porous graphite
BAIOCCHI, Claudio;PAZZI, Marco;MEDANA, Claudio;
2003-01-01
Abstract
A method is presented for the separation and detection of eight synthetic corticosteroids in bovine liver tissues. Following sample preparation, the samples were separated by isocratic LC (mobile phase: 85% methanol, 15% dichloromethane) using a porous graphite (PGC) column and detected by ion trap MS-MS, using an atmospheric pressure chemical ionization (APCI) source and monitoring positive ions. An octaclecyl-silica phase system was also used for comparison. With the PGC stationary phase the critical isomer separation of dexamethasone and betamethasone was achieved. The performances of MS-MS, MS3, selected ion monitoring (SIM) and selected reaction monitoring (SRM) detection modes were checked and best conditions of sensitivity were obtained in MS-MS mode.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
