The application of AFLP, STMS and EST markers to study the genetic relationships in an evergreen azalea gene pool was investigated. STMS and EST markers revealed a higher genetic distance detection capacity than AFLPs, which, nevertheless were the most efficient marker system due to their highest polymorphism detection capacity. Similarity matrices showed weak, yet significant, correlations when Mantel’s test was applied. To assess the usefulness of the overall information provided by these marker data for establishing phylogenetic relationships and horticultural classification, cluster analysis was performed. The joined AFLP, STMS and EST data were demonstrated to be remarkably effective for group discrimination and phylogenetic studies. The use of these PCR-marker systems is discussed in terms of the choice of appropriate marker technique for different aspects of evergreen azalea germplasm evaluation.

Comparative study of the discriminating capacity and effectiveness in assessing genetic relationships of AFLP, STMS and EST markers in evergreen azaleas

SCARIOT, VALENTINA;
2007-01-01

Abstract

The application of AFLP, STMS and EST markers to study the genetic relationships in an evergreen azalea gene pool was investigated. STMS and EST markers revealed a higher genetic distance detection capacity than AFLPs, which, nevertheless were the most efficient marker system due to their highest polymorphism detection capacity. Similarity matrices showed weak, yet significant, correlations when Mantel’s test was applied. To assess the usefulness of the overall information provided by these marker data for establishing phylogenetic relationships and horticultural classification, cluster analysis was performed. The joined AFLP, STMS and EST data were demonstrated to be remarkably effective for group discrimination and phylogenetic studies. The use of these PCR-marker systems is discussed in terms of the choice of appropriate marker technique for different aspects of evergreen azalea germplasm evaluation.
2007
126
207
212
Rhododendron spp.; Tsutsusi; Ericaceae; phylogeny; marker comparisons; expressed sequence tags; microsatellites; sequence tagged sites
V. SCARIOT; E. DE KEYSER; T. HANDA; J. DE RIEK
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/23176
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