The application of AFLP, STMS and EST markers to study the genetic relationships in an evergreen azalea gene pool was investigated. STMS and EST markers revealed a higher genetic distance detection capacity than AFLPs, which, nevertheless were the most efficient marker system due to their highest polymorphism detection capacity. Similarity matrices showed weak, yet significant, correlations when Mantel’s test was applied. To assess the usefulness of the overall information provided by these marker data for establishing phylogenetic relationships and horticultural classification, cluster analysis was performed. The joined AFLP, STMS and EST data were demonstrated to be remarkably effective for group discrimination and phylogenetic studies. The use of these PCR-marker systems is discussed in terms of the choice of appropriate marker technique for different aspects of evergreen azalea germplasm evaluation.
Comparative study of the discriminating capacity and effectiveness in assessing genetic relationships of AFLP, STMS and EST markers in evergreen azaleas
SCARIOT, VALENTINA;
2007-01-01
Abstract
The application of AFLP, STMS and EST markers to study the genetic relationships in an evergreen azalea gene pool was investigated. STMS and EST markers revealed a higher genetic distance detection capacity than AFLPs, which, nevertheless were the most efficient marker system due to their highest polymorphism detection capacity. Similarity matrices showed weak, yet significant, correlations when Mantel’s test was applied. To assess the usefulness of the overall information provided by these marker data for establishing phylogenetic relationships and horticultural classification, cluster analysis was performed. The joined AFLP, STMS and EST data were demonstrated to be remarkably effective for group discrimination and phylogenetic studies. The use of these PCR-marker systems is discussed in terms of the choice of appropriate marker technique for different aspects of evergreen azalea germplasm evaluation.File | Dimensione | Formato | |
---|---|---|---|
Scariot_azalea_plant breeding.pdf
Accesso riservato
Tipo di file:
POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione
527.58 kB
Formato
Adobe PDF
|
527.58 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.