Groups of mice were immunized intraperitoneally with HEp-2 lysate or respiratory syncytial virus (RSV)-infected HEp-2 cells with or without adjuvant(s). The animals were subsequently challenged intranasally (i.n.) with RSV and/or HEp-2 lysate and studied for RSV shedding in the lung, antigen-specific antibody response and pulmonary histopathology. A significant decrease in virus shedding was detected in each of four groups of animals immunized with a virus preparation and in two of four groups that received a HEp-2 lysate. Mice immunized with adjuvant(s) developed higher antibody-specific responses. All vaccinated animals developed pulmonary histopathology only on subsequent i.n. inoculation with RSV and/or HEp-2 lysate. Denatured extracts of purified RSV, HEp-2, BALB/c lung, cotton rat lung, Buffalo green monkey kidney and human buccal epithelium were tested for reactivity against the sera of immunized mice by an immunoblot method. Sera from all groups of immunized mice reacted with the extracts tested. The data suggest a possible role of reactivity to viral as well as non-viral components in the pathogenesis of RSV vaccine-induced pulmonary inflammation in the mouse model system.
Immune response to experimentally induced infection with respiratory syncytial virus: possible role in the development of pulmonary disease.
CAMUSSI, Giovanni;
1989-01-01
Abstract
Groups of mice were immunized intraperitoneally with HEp-2 lysate or respiratory syncytial virus (RSV)-infected HEp-2 cells with or without adjuvant(s). The animals were subsequently challenged intranasally (i.n.) with RSV and/or HEp-2 lysate and studied for RSV shedding in the lung, antigen-specific antibody response and pulmonary histopathology. A significant decrease in virus shedding was detected in each of four groups of animals immunized with a virus preparation and in two of four groups that received a HEp-2 lysate. Mice immunized with adjuvant(s) developed higher antibody-specific responses. All vaccinated animals developed pulmonary histopathology only on subsequent i.n. inoculation with RSV and/or HEp-2 lysate. Denatured extracts of purified RSV, HEp-2, BALB/c lung, cotton rat lung, Buffalo green monkey kidney and human buccal epithelium were tested for reactivity against the sera of immunized mice by an immunoblot method. Sera from all groups of immunized mice reacted with the extracts tested. The data suggest a possible role of reactivity to viral as well as non-viral components in the pathogenesis of RSV vaccine-induced pulmonary inflammation in the mouse model system.
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