In this paper the Authors describe a new method they have adopted (in studies reported in accompanying papers II and III) for measuring S-adenosylmethionine decarboxylase activity in entire cells and cell fractions. The new method is a general one for recovering and measuring labelled carbon dioxide formed 'in vitro' during enzymatic reactions. It presents several advantages when compared to traditional methods.
Effect of aldehydes on polyamine metabolism. I. Method used to determine CO2 produced 'in vitro' in enzymatic reactions: its application in evaluation of S-adenosylmethionine decarboxylase (SAMD) activity.
CHIARPOTTO, Elena Maria;POLI, Giuseppe;
1981-01-01
Abstract
In this paper the Authors describe a new method they have adopted (in studies reported in accompanying papers II and III) for measuring S-adenosylmethionine decarboxylase activity in entire cells and cell fractions. The new method is a general one for recovering and measuring labelled carbon dioxide formed 'in vitro' during enzymatic reactions. It presents several advantages when compared to traditional methods.
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