Seven alloantisera were selected showing the following characteristics: after platelet absorption they were positive with PHA-activated cells and negative with resting T and B lymphocytes from the same donors; this reactivity could be blocked by pretreatment of cells with a turkey anti-human beta 2-microglobulin serum and was not completely removed by further absorption with high doses of pooled platelets or with T or B lymphocytes from the positive donors; the reactivity of some of these antisera correlated with HLA-A antigens in a panel of PHA-activated cells and segregated with HLA haplotypes in families. It is still to be proven if the HLA determinants recognized on PHA-activated cells by these antisera are new epitopes of A,B,C molecules appearing upon activation or if they are carried by products of a new polymorphic locus linked to the HLA system.

Alloantisera with apparent HLA-A specificity reacting only against phytohemagglutinin-activated cells. / CREPALDI T ;AMOROSO A ;CURTONI ES ;RICHIARDI P. - In: LA RICERCA IN CLINICA E IN LABORATORIO. - ISSN 0390-5748. - 16(1986), pp. 449-456.

Alloantisera with apparent HLA-A specificity reacting only against phytohemagglutinin-activated cells.

CREPALDI, Tiziana;AMOROSO, Antonio;
1986

Abstract

Seven alloantisera were selected showing the following characteristics: after platelet absorption they were positive with PHA-activated cells and negative with resting T and B lymphocytes from the same donors; this reactivity could be blocked by pretreatment of cells with a turkey anti-human beta 2-microglobulin serum and was not completely removed by further absorption with high doses of pooled platelets or with T or B lymphocytes from the positive donors; the reactivity of some of these antisera correlated with HLA-A antigens in a panel of PHA-activated cells and segregated with HLA haplotypes in families. It is still to be proven if the HLA determinants recognized on PHA-activated cells by these antisera are new epitopes of A,B,C molecules appearing upon activation or if they are carried by products of a new polymorphic locus linked to the HLA system.
16
449
456
CREPALDI T ;AMOROSO A ;CURTONI ES ;RICHIARDI P
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/30811
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