Sera from rubella patients were fractionated with six different gels on a 0.9 X 30 cm column. Fractionation efficiency was evaluated by determining the IgM, IgA and IgG concentrations in each fraction and titring the rubella virus hemagglutination-inhibiting antibodies before and after treatment with 2-mercaptoethanol. All six gels were able to separate IgM from the other Ig, but the distance between their elution peaks varied considerably. Assessment of the usefulness of a gel for diagnostic purposes requires evaluation of its ability to separate IgM from the oligometric IgA often present in sera and a possible source of diagnostic error. Ultrogel AcA 22 and Bio-Gel A5m gave the best results. In addition to displaying good peak separation, they eluted IgM within their linear fractionation range.

Separation of IgM for diagnostic purposes by exclusion chromatography: a comparison between six gels. / ANGERETTI A ;MERLINO C ;NEGRO PONZI A. - In: BOLLETTINO DELL'ISTITUTO SIEROTERAPICO MILANESE. - ISSN 0021-2547. - 62(1983), pp. 501-508.

Separation of IgM for diagnostic purposes by exclusion chromatography: a comparison between six gels.

MERLINO, Chiara;
1983

Abstract

Sera from rubella patients were fractionated with six different gels on a 0.9 X 30 cm column. Fractionation efficiency was evaluated by determining the IgM, IgA and IgG concentrations in each fraction and titring the rubella virus hemagglutination-inhibiting antibodies before and after treatment with 2-mercaptoethanol. All six gels were able to separate IgM from the other Ig, but the distance between their elution peaks varied considerably. Assessment of the usefulness of a gel for diagnostic purposes requires evaluation of its ability to separate IgM from the oligometric IgA often present in sera and a possible source of diagnostic error. Ultrogel AcA 22 and Bio-Gel A5m gave the best results. In addition to displaying good peak separation, they eluted IgM within their linear fractionation range.
62
501
508
ANGERETTI A ;MERLINO C ;NEGRO PONZI A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/30989
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