High flow rates permit drastic reduction of time required in human serum fractionation by gel filtration giving relatively pure IgM. Fractionation of human serum on Bio-Gel A5m, Superose 6B and Sephacryl S-400, packed in 0.9 X 30 cm columns, at flow rate of 24 cm/h gives IgM fractions employable in diagnosis, in one hour time only.
[Serum fractionation by gel filtration at a high flow rate: use for diagnostic purposes]
MERLINO, Chiara;
1983-01-01
Abstract
High flow rates permit drastic reduction of time required in human serum fractionation by gel filtration giving relatively pure IgM. Fractionation of human serum on Bio-Gel A5m, Superose 6B and Sephacryl S-400, packed in 0.9 X 30 cm columns, at flow rate of 24 cm/h gives IgM fractions employable in diagnosis, in one hour time only.
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