The enhanced sorbitol synthesis in diabetic red blood cells can lead to a depletion of NADPH and, by limiting the amount of GSH produced in the glutathione-reductase step, can make the cell more susceptible to oxidant injury. Diabetic red blood cells have a shortened life span, as shown by the increase of creatine level. The osmotic resistance of diabetic erythrocytes was measured and the erythrocyte flexibility was studied using a filtration method in which the cells were forced, at a constant flow rate, through a polycarbonate membrane with 5-mu pores. The increased osmotic resistance and the impaired flexibility shown by diabetic red blood cells can be induced in normal cells by a treatment with SH-reagents (diamide). This points to a redox state-mediated decrease in deformability and survival of diabetic erythrocytes.

[Changes in erythrocyte deformability following changes in oxido-reductive metabolism in diabetes mellitus] / GHIGO D ;BOSIA A ;PAGANI A ;PESCARMONA GP ;PAGANO G ;LENTI G. - In: LA RICERCA IN CLINICA E IN LABORATORIO. - ISSN 0390-5748. - 13 Suppl 3(1983), pp. 375-381.

[Changes in erythrocyte deformability following changes in oxido-reductive metabolism in diabetes mellitus]

GHIGO, Dario Antonio;BOSIA, Amalia;PESCARMONA, Gianpiero;
1983

Abstract

The enhanced sorbitol synthesis in diabetic red blood cells can lead to a depletion of NADPH and, by limiting the amount of GSH produced in the glutathione-reductase step, can make the cell more susceptible to oxidant injury. Diabetic red blood cells have a shortened life span, as shown by the increase of creatine level. The osmotic resistance of diabetic erythrocytes was measured and the erythrocyte flexibility was studied using a filtration method in which the cells were forced, at a constant flow rate, through a polycarbonate membrane with 5-mu pores. The increased osmotic resistance and the impaired flexibility shown by diabetic red blood cells can be induced in normal cells by a treatment with SH-reagents (diamide). This points to a redox state-mediated decrease in deformability and survival of diabetic erythrocytes.
13 Suppl 3
375
381
GHIGO D ;BOSIA A ;PAGANI A ;PESCARMONA GP ;PAGANO G ;LENTI G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/32157
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