The use of a self-contained unit-gravity cell separation apparatus for separation of populations of mouse testicular cells is described. The apparatus, a Celsep (TM), maximizes the unit area over which sedimentation occurs, reduces the amount of separation medium employed, and is quite reproducible. Cells thus isolated have been good sources for isolation of DNA, and notably, high molecular weight RNA.
Separation of mouse testis cells on a Celsep (TM) apparatus and their usefulness as a source of high molecular weight DNA or RNA. / WOLGEMUTH DJ ;GIZANG-GINSBERG E ;ENGELMYER E ;GAVIN BJ ;PONZETTO C. - In: GAMETE RESEARCH. - ISSN 0148-7280. - 12(1985), pp. 1-10.
Titolo: | Separation of mouse testis cells on a Celsep (TM) apparatus and their usefulness as a source of high molecular weight DNA or RNA. |
Autori Riconosciuti: | |
Autori: | WOLGEMUTH DJ ;GIZANG-GINSBERG E ;ENGELMYER E ;GAVIN BJ ;PONZETTO C |
Data di pubblicazione: | 1985 |
Abstract: | The use of a self-contained unit-gravity cell separation apparatus for separation of populations of mouse testicular cells is described. The apparatus, a Celsep (TM), maximizes the unit area over which sedimentation occurs, reduces the amount of separation medium employed, and is quite reproducible. Cells thus isolated have been good sources for isolation of DNA, and notably, high molecular weight RNA. |
Volume: | 12 |
Pagina iniziale: | 1 |
Pagina finale: | 10 |
Rivista: | GAMETE RESEARCH |
Appare nelle tipologie: | 03A-Articolo su Rivista |