Electrophoresis in cellulose acetate in the presence of 3% Nonidet P-40 can resolve two neutral genetic variants, A gamma and G gamma human fetal globin chains. The ratio of these two chains, determined by densitometry of the electrophoretic strips, is in excellent agreement with the Gly-Ala ratio obtained by chemical analysis of the cyanogen bromide fragment gamma CB3. It is suggested that the detergent binds preferentially to the hydrophobic amino acid segment 133-141 in the A gamma chain, thus masking either a Lys or an Arg residue at the two extremes.

Electrophoretic separation of A gamma and G gamma human globin chains in Nonidet P-40.

GUERRASIO, Angelo;SAGLIO, Giuseppe;
1979-01-01

Abstract

Electrophoresis in cellulose acetate in the presence of 3% Nonidet P-40 can resolve two neutral genetic variants, A gamma and G gamma human fetal globin chains. The ratio of these two chains, determined by densitometry of the electrophoretic strips, is in excellent agreement with the Gly-Ala ratio obtained by chemical analysis of the cyanogen bromide fragment gamma CB3. It is suggested that the detergent binds preferentially to the hydrophobic amino acid segment 133-141 in the A gamma chain, thus masking either a Lys or an Arg residue at the two extremes.
1979
99
7
11
GUERRASIO A ;SAGLIO G ;MAZZA U ;PICH P ;CAMASCHELLA C ;RICCO G ;GIANAZZA E ;RIGHETTI PG
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/35071
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