The Hu family of RNA-binding proteins is involved in many post-transcriptional mechanisms for the development and maintenance of the nervous system. Three members of the Hu family (HuB, HuC and HuD) are neuron-specific proteins. In this study, we present data using light and electron microscopy to show the sub-cellular localization of neuron-specific Hu proteins in rat primary sensory neurons taken from dorsal root ganglia (DRG). Using these techniques we morphologically revealed the presence of neuron-specific-Hu proteins in the nucleus and in the cytoplasm and discriminated the presence of Hu proteins within different cellular organelles, specifically mitochondria and Golgi apparatus, thus supporting previous ideas that NS-Hu proteins enable RNA interactions with sub-cellular organelles and may be involved in mRNA cellular localization.
Neuron-specific Hu proteins sub-cellular localization in primary sensory neurons
FORNARO, Michele;RAIMONDO, Stefania;ROBECCHI, Mariagiuseppina
2007-01-01
Abstract
The Hu family of RNA-binding proteins is involved in many post-transcriptional mechanisms for the development and maintenance of the nervous system. Three members of the Hu family (HuB, HuC and HuD) are neuron-specific proteins. In this study, we present data using light and electron microscopy to show the sub-cellular localization of neuron-specific Hu proteins in rat primary sensory neurons taken from dorsal root ganglia (DRG). Using these techniques we morphologically revealed the presence of neuron-specific-Hu proteins in the nucleus and in the cytoplasm and discriminated the presence of Hu proteins within different cellular organelles, specifically mitochondria and Golgi apparatus, thus supporting previous ideas that NS-Hu proteins enable RNA interactions with sub-cellular organelles and may be involved in mRNA cellular localization.
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