An easy and cheap biosensor has been developed for assessing soil biohazards. The toxicity assay is based on inhibition of Dictyostelium development, a soil amoeba undergoing multicellular development and cell differentiation under starving conditions. The sensitivity of the assay was assessed in soil samples with increasing concentrations of heavy metals and by comparison with standard bio-assays on a battery of soils collected from con-taminated industrial sites. Dictyostelium appears to be highly sensitive to polycyclic aromatic hydrocarbons, mineral oil and, to a lesser extent, to heavy metals. The assay has been applied in a concerted action with other bioassays within the frame of the BIO-BIO project.
A novel biossay for evaluating soil bio-hazards using dictyostelium as biosensor: validation and application to the bio-bio project
BOZZARO, Salvatore
2008-01-01
Abstract
An easy and cheap biosensor has been developed for assessing soil biohazards. The toxicity assay is based on inhibition of Dictyostelium development, a soil amoeba undergoing multicellular development and cell differentiation under starving conditions. The sensitivity of the assay was assessed in soil samples with increasing concentrations of heavy metals and by comparison with standard bio-assays on a battery of soils collected from con-taminated industrial sites. Dictyostelium appears to be highly sensitive to polycyclic aromatic hydrocarbons, mineral oil and, to a lesser extent, to heavy metals. The assay has been applied in a concerted action with other bioassays within the frame of the BIO-BIO project.
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