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EnglishVarious enzymatic or mechanical methods have been proposed in the past to dissociate cells from different solid tissues. An automated mechanical disaggregation device (Medimachine) has recently been proposed. Unfortunately, most of these techniques are associated with a high cellular damage and a low cell recovery and are difficult to apply to skin biopsies. In this paper, we propose a combined enzymatic and mechanical method based on Medimachine, useful for the isolation of skin infiltrating T-lymphocytes from small cutaneous biopsies. As this method is easy and allows for a more correct qualitative and quantitative cytofluorimetric analysis of the lymphocyte subsets, it may be useful in the immunophenotyping of cutaneous T-cell lymphomas.
| Titolo: | Collagenase digestion and mechanical disaggregation as a method to extract and immunophenotype tumour lymphocytes in cutaneous T-cell lymphomas. |
| Autori Riconosciuti: | |
| Autori: | M. NOVELLI; SAVOIA P; CAMBIERI I; PONTI R; COMESSATTI A; LISA F; BERNENGO MG |
| Data di pubblicazione: | 2000 |
| Abstract: | Various enzymatic or mechanical methods have been proposed in the past to dissociate cells from different solid tissues. An automated mechanical disaggregation device (Medimachine) has recently been proposed. Unfortunately, most of these techniques are associated with a high cellular damage and a low cell recovery and are difficult to apply to skin biopsies. In this paper, we propose a combined enzymatic and mechanical method based on Medimachine, useful for the isolation of skin infiltrating T-lymphocytes from small cutaneous biopsies. As this method is easy and allows for a more correct qualitative and quantitative cytofluorimetric analysis of the lymphocyte subsets, it may be useful in the immunophenotyping of cutaneous T-cell lymphomas. |
| Volume: | 25 |
| Pagina iniziale: | 423 |
| Pagina finale: | 431 |
| Digital Object Identifier (DOI): | 10.1046/j.1365-2230.2000.00680.x |
| Rivista: | CLINICAL AND EXPERIMENTAL DERMATOLOGY |
| Appare nelle tipologie: | 03A-Articolo su Rivista |
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