The asbestiform fibrous silicate balangeroite exhibits cytotoxic and oxidative properties similar to those exerted by crocidolite asbestos. In human lung epithelial cells A549, balangeroite, like crocidolite, inhibited the pentose phosphate pathway (PPP), one of the main antioxidant intracellular tools; this inhibition was exerted also when PPP was activated by the redox-cycling compound menadione. PPP inhibition may be accounted for by the inhibition of its rate-limiting enzyme, glucose-6-phosphate dehydrogenase (G6PD). Reduced glutathione (GSH), the most important intracellular antioxidant molecule, was decreased by both balangeroite and crocidolite incubation. This effect was not related to any increased content of oxidized glutathione, or to any enhanced efflux of glutathione, suggesting that balangeroite fibers, like crocidolite, might favor the reaction of GSH with other molecules.

Potential toxicity of nonregulated asbestiform minerals: balangeroite from the western Alps. Part 3: Depletion of antioxidant defenses

GAZZANO, Elena;RIGANTI, Chiara;TOMATIS, Maura;TURCI, Francesco;BOSIA, Amalia;FUBINI, Bice;GHIGO, Dario Antonio
2005-01-01

Abstract

The asbestiform fibrous silicate balangeroite exhibits cytotoxic and oxidative properties similar to those exerted by crocidolite asbestos. In human lung epithelial cells A549, balangeroite, like crocidolite, inhibited the pentose phosphate pathway (PPP), one of the main antioxidant intracellular tools; this inhibition was exerted also when PPP was activated by the redox-cycling compound menadione. PPP inhibition may be accounted for by the inhibition of its rate-limiting enzyme, glucose-6-phosphate dehydrogenase (G6PD). Reduced glutathione (GSH), the most important intracellular antioxidant molecule, was decreased by both balangeroite and crocidolite incubation. This effect was not related to any increased content of oxidized glutathione, or to any enhanced efflux of glutathione, suggesting that balangeroite fibers, like crocidolite, might favor the reaction of GSH with other molecules.
2005
68
41
49
balangeroite; glutathione; pentose phosphate pathway; glucose-6-phopshate dehydrogenase
Gazzano E; Riganti C; Tomatis M; Turci F; Bosia A; Fubini B; Ghigo D
File in questo prodotto:
File Dimensione Formato  
Gazzano, JTEH, 2005.pdf

Accesso riservato

Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 120.48 kB
Formato Adobe PDF
120.48 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/42582
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 38
  • ???jsp.display-item.citation.isi??? 37
social impact