We report-herein the labeling of 17alpha-ethynyl-17beta-estradiol with a ''Co3(CO)9'' fragment. The cluster exhibits fully reversible electrochemical behavior and is assayed by means of the square-wave voltammetric technique. During the condensation of metallocarbonyl fragments around the organic chain, elimination of the 17beta-OH group in the original molecule occurs, affording Co3(CO)9(dehydroxyethynylestradiol). This modification causes an important decrease of relative binding affinity for the receptors. However, the cluster can still be used for immunoassay of estradiol, since its cross reactivity for specific antibodies is high.

Estrogen Derivatives Of Transition-Metal Complexes For Analytical Detection Enhancement

OSELLA, Domenico;GAMBINO, Olimpia;NERVI, Carlo;
1994

Abstract

We report-herein the labeling of 17alpha-ethynyl-17beta-estradiol with a ''Co3(CO)9'' fragment. The cluster exhibits fully reversible electrochemical behavior and is assayed by means of the square-wave voltammetric technique. During the condensation of metallocarbonyl fragments around the organic chain, elimination of the 17beta-OH group in the original molecule occurs, affording Co3(CO)9(dehydroxyethynylestradiol). This modification causes an important decrease of relative binding affinity for the receptors. However, the cluster can still be used for immunoassay of estradiol, since its cross reactivity for specific antibodies is high.
13
3110
3114
CLUSTER COMPLEXES; TRICOBALT CARBON; IMMUNOASSAY; ESTRADIOL
D. OSELLA; O. GAMBINO; C. NERVI; E. STEIN; G. JAOUEN; A. VESSIERES
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/44524
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 9
social impact