The aim of the work was to provide information useful in the discrimination of cultivars and ecotypes of runner bean (Phaseolus coccineus L.). Samples of about 100 plants from 15 genotypes were analyzed for isozyme variants in 19 enzyme systems, by means of starch gel electrophoresis. Only two isozymes, alcohol dehydrogenase and shikimic dehydrogenase, were polymorphic and consistent enough to be used in the study. Genotypes were pairwise compared by means of allelic frequencies, since they were seen to agree with Hardy-Weinberg proportions. The two considered loci showed a different reliability in the differentiation of the genotypes by means of their allelic frequencies and their combined evaluation allowed us to distinguish more than 92% comparisons. This value was increased by taking into consideration one morphological marker such as the colour of the seed testa. The results of the study show the potentiality of isozyme analysis for use in distinguishing closely related genotypes of runner bean.

Identification of runner bena genotypes (Phaseolus coccineus L.) by means of isozyme analysis.

BELLETTI, Piero;
1996-01-01

Abstract

The aim of the work was to provide information useful in the discrimination of cultivars and ecotypes of runner bean (Phaseolus coccineus L.). Samples of about 100 plants from 15 genotypes were analyzed for isozyme variants in 19 enzyme systems, by means of starch gel electrophoresis. Only two isozymes, alcohol dehydrogenase and shikimic dehydrogenase, were polymorphic and consistent enough to be used in the study. Genotypes were pairwise compared by means of allelic frequencies, since they were seen to agree with Hardy-Weinberg proportions. The two considered loci showed a different reliability in the differentiation of the genotypes by means of their allelic frequencies and their combined evaluation allowed us to distinguish more than 92% comparisons. This value was increased by taking into consideration one morphological marker such as the colour of the seed testa. The results of the study show the potentiality of isozyme analysis for use in distinguishing closely related genotypes of runner bean.
1996
50
185
190
Allelic frequency; Cultivar identification; Isozymes; Runner bean; Phaseolus coccineus; Starch gel electrophoresis
P. BELLETTI; S. LOTITO
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/46659
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