A new glycol methacrylate (GMA) polar resin technique was used for light microscopy and histochemistry of Macrotrachela quadricornifera. Animals were treated with cold aqueous solution of ethylene glycol (EG), then embedded in the cold. Animals required non conventional chemical fixation as EG stabilizes, dehydrated and cryopreserves their structure. In this way several enzimatic activities are preserved, along with morphology. Moreover, resin reticulate protect tissues against agressive reagents. As a consequence, it is possible to perform different staining procedures, in sequence, on the same section.
Polar resin embedding for Machrotrachela quadricornifera
DORE, Bruno Emilio;
1993-01-01
Abstract
A new glycol methacrylate (GMA) polar resin technique was used for light microscopy and histochemistry of Macrotrachela quadricornifera. Animals were treated with cold aqueous solution of ethylene glycol (EG), then embedded in the cold. Animals required non conventional chemical fixation as EG stabilizes, dehydrated and cryopreserves their structure. In this way several enzimatic activities are preserved, along with morphology. Moreover, resin reticulate protect tissues against agressive reagents. As a consequence, it is possible to perform different staining procedures, in sequence, on the same section.
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