Using a new "cryostabilization" histotechnique, avoiding any chemical fixation, and then cold embedding in a polar plastic (Technovit 7100, Kulzer), we were successfully in revealing aspecific alkaline phosphatase activity also in the cytoplasm of some tracts of Xenopus laevis proximal convoluted tubule (p.c.t.). On the basis of this and other histochemical reactions, we thus can identify three different tracts in the p.c.t., previously considered uniform.
Identificazione istochimica dell'attività fosfatasica alcalina aspecifica citoplasmatica nelle cellule di alcuni tratti del convoluto prossimale del rene di Xenopus laevis
DORE, Bruno Emilio;USAI, Pasquale
1999-01-01
Abstract
Using a new "cryostabilization" histotechnique, avoiding any chemical fixation, and then cold embedding in a polar plastic (Technovit 7100, Kulzer), we were successfully in revealing aspecific alkaline phosphatase activity also in the cytoplasm of some tracts of Xenopus laevis proximal convoluted tubule (p.c.t.). On the basis of this and other histochemical reactions, we thus can identify three different tracts in the p.c.t., previously considered uniform.
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