1 Triphenyltin acetate (TPTA) has been shown to exert in vivo a selective toxic effect on the immune system. To assess in vitro possible alterations induced by TPTA exposure, primary cultures of mouse thymocytes were incubated up to 24 h with graded amounts (1 - 12 mu M) of the organotin. 2 The cytotoxic activity has been evaluated with the MTT colorimetric assay, the neutral red (NR) assay and the lactic dehydrogenase (LDH) cellular release. Cell pellets were fixed with 2.5% glutaraldehyde, resin-embedded and ultrathin sections were observed through transmission electron microscopy. 3 After 2 h of incubation, dose-dependent increases of cytotoxicity were observed in thymocytes submitted to MTT and NR tests (up to 41.43% and 18.9%, respectively), while 22 h later this overt effect on cell viability was noticed merely in cells exposed to 12 mu M TPTA. Dose-dependent increases of LDH leakage in the culture medium were observed all throughout the study. 4 Morphological investigations revealed features (chromatin condensation, cell membranes fragmentation and formation of membrane bound apoptotic bodies) suggestive of apoptosis. 5 This study indicates that TPTA is cytotoxic to mouse thymocytes: morphologically, the rising of apoptosis is likely to be recognized, as previously reported in different in vitro studies with other immunosuppressive agents as dioxin and corticosteroids.

Triphenyltin acetate toxicity: a biochemical and ultrastructural study on mouse thymocytes.

BOLLO, Enrico;CEPPA, Leonardo;CORNAGLIA, Enzo;NEBBIA, Carlo;BIOLATTI, Bartolomeo;DACASTO, Mauro
1996

Abstract

1 Triphenyltin acetate (TPTA) has been shown to exert in vivo a selective toxic effect on the immune system. To assess in vitro possible alterations induced by TPTA exposure, primary cultures of mouse thymocytes were incubated up to 24 h with graded amounts (1 - 12 mu M) of the organotin. 2 The cytotoxic activity has been evaluated with the MTT colorimetric assay, the neutral red (NR) assay and the lactic dehydrogenase (LDH) cellular release. Cell pellets were fixed with 2.5% glutaraldehyde, resin-embedded and ultrathin sections were observed through transmission electron microscopy. 3 After 2 h of incubation, dose-dependent increases of cytotoxicity were observed in thymocytes submitted to MTT and NR tests (up to 41.43% and 18.9%, respectively), while 22 h later this overt effect on cell viability was noticed merely in cells exposed to 12 mu M TPTA. Dose-dependent increases of LDH leakage in the culture medium were observed all throughout the study. 4 Morphological investigations revealed features (chromatin condensation, cell membranes fragmentation and formation of membrane bound apoptotic bodies) suggestive of apoptosis. 5 This study indicates that TPTA is cytotoxic to mouse thymocytes: morphologically, the rising of apoptosis is likely to be recognized, as previously reported in different in vitro studies with other immunosuppressive agents as dioxin and corticosteroids.
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219
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mouse; lymphocyte; triphenyltin acetate; apoptosis
Bollo E; Ceppa L; Cornaglia E; Nebbia C; Biolatti B; Dacasto M.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/53668
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