Ouabain, a specific inhibitor of the membrane Na+K+ATPase, is known to inhibit the proliferation of human lymphocytes in culture induced by any of a variety of agents. Here we show that the cytotoxic activity of human lymphocytes precultured with ouabain for 1 day against antobody coated target cells (ADCC) was inhibited in a concentration dependent manner. Ouabain acted synergistically with the respiratory chain inhibitor antimicin A to block killing, but did not act synegistically with the competitive inhibitor of glucose utilization 2.deoxyglucose. T-cell cytotoxicity was more sensitive to ouabain than was ADCC.
Ouabain inhibition of Human Lymphocyte Cytotoxicity
DE MARCHI, Mario
1979-01-01
Abstract
Ouabain, a specific inhibitor of the membrane Na+K+ATPase, is known to inhibit the proliferation of human lymphocytes in culture induced by any of a variety of agents. Here we show that the cytotoxic activity of human lymphocytes precultured with ouabain for 1 day against antobody coated target cells (ADCC) was inhibited in a concentration dependent manner. Ouabain acted synergistically with the respiratory chain inhibitor antimicin A to block killing, but did not act synegistically with the competitive inhibitor of glucose utilization 2.deoxyglucose. T-cell cytotoxicity was more sensitive to ouabain than was ADCC.
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