A sample of about 169 tomato landraces of varying fruit type and of diverse provenance was tested for reaction to infection with Verticillium dahliae, the causative pathogen of Verticillium wilt. Some individuals of the ‘Sorrentino’ landrace were as resistant as ‘Florida Ve’, whereas certain populations of the ‘Ligure’, rather known as ‘Cuor di bue di Albenga’, type included the extremes of susceptibility and resistance. Resistant selections from each landrace were allowed to self fertilize to generate breeding lines. A PCR analysis, based on a CAPS marker targeted to the Ve2 allele, was conducted on ‘Sorrentino’, ‘Locale di Albenga 2’, ‘Locale di Albenga 3’, ‘Locale di Carmagnola’, ‘Locale di Cuneo’, and ‘Eraldo4’, and the resulting genotype was fully consistent with reaction to infection with V. dahliae strain V7. Variation in the level of resistance among Ve2 carriers suggested that other genes are also involved in modulating the response to infection. Markers for resistant alleles at Ve should prove useful in marker assisted breeding for wilt resistance in tomato. Homozygous resistant selections were made from landrace materials, which retained the landrace fruit type.
The Presence of Genes for Resistance against Verticillium dahliae in Italian Tomato Landraces
VALENTINO, Danila;TAMIETTI, Giacomo
2010-01-01
Abstract
A sample of about 169 tomato landraces of varying fruit type and of diverse provenance was tested for reaction to infection with Verticillium dahliae, the causative pathogen of Verticillium wilt. Some individuals of the ‘Sorrentino’ landrace were as resistant as ‘Florida Ve’, whereas certain populations of the ‘Ligure’, rather known as ‘Cuor di bue di Albenga’, type included the extremes of susceptibility and resistance. Resistant selections from each landrace were allowed to self fertilize to generate breeding lines. A PCR analysis, based on a CAPS marker targeted to the Ve2 allele, was conducted on ‘Sorrentino’, ‘Locale di Albenga 2’, ‘Locale di Albenga 3’, ‘Locale di Carmagnola’, ‘Locale di Cuneo’, and ‘Eraldo4’, and the resulting genotype was fully consistent with reaction to infection with V. dahliae strain V7. Variation in the level of resistance among Ve2 carriers suggested that other genes are also involved in modulating the response to infection. Markers for resistant alleles at Ve should prove useful in marker assisted breeding for wilt resistance in tomato. Homozygous resistant selections were made from landrace materials, which retained the landrace fruit type.
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