Since its introduction as an electrondense marker for electron microscopy in 1971, colloidal gold has become the marker of choice for the in situ detection of a huge number of cellular and extracellular molecules by transmission electron microscopy. We will review here the basic post-embedding immunogold and immunogold-silver labeling methods, and then discuss some more recent applications based on the combination of colloidal gold immunocytochemistry with enzyme histochemistry, tracing techniques, and transferase-immunogold labeling procedures for the use in cell and molecular biology.
Immunogold labeling for transmission electron microscopy: exploring new frontiers.
LOSSI, Laura;MERIGHI, Adalberto
1997-01-01
Abstract
Since its introduction as an electrondense marker for electron microscopy in 1971, colloidal gold has become the marker of choice for the in situ detection of a huge number of cellular and extracellular molecules by transmission electron microscopy. We will review here the basic post-embedding immunogold and immunogold-silver labeling methods, and then discuss some more recent applications based on the combination of colloidal gold immunocytochemistry with enzyme histochemistry, tracing techniques, and transferase-immunogold labeling procedures for the use in cell and molecular biology.
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