Objective: Steroid-producing-cell autoantibodies (SCA) directed against 21-hydroxylase (21OHAb), 17α-hydroxylase (17OHAb) and cholesterol side-chain-cleavage enzyme (P450sccAb), characterize autoimmune primary ovarian insufficiency (SCA-POI). The aim of the study was to analyze IgG-subclass specificity of autoantibodies related to adrenal and ovarian autoimmunity. Design: We studied 29 women with SCA-POI, 30 women with autoimmune Addison disease (AAD) without POI and 14 patients with autoimmune polyendocrine syndrome type 1 (APS1). 21OHAb isotypes were also analyzed in 14 subjects with pre-clinical AAD. Samples from 30 healthy women served as control group to determine upper level of normality in the isotype assays. Methods: Immunoradiometric assays with IgG-subclass-specific secondary antibodies. Results: In 21OHAb-positive sera, IgG1 isotype was detected in 90% SCA-POI and non-POI AAD and 67% APS 1 patients. IgG1 isotype was found in 69% 17OHAb-positive SCA-POI and 100% 17OHAb-positive APS1 sera and in 60% P450sccAb-positive SCA-POI and 80% P450sccAb-positive APS1 sera. For 21OHAb, IgG4 isotype was detected in 17% SCA-POI, 7% non-POI AAD and 8% APS1 sera. None of the 17OHAb-positive sera was positive for IgG4. In P450sccAb-positive sera, 15% aPOI and 20% APS1 sera were positive for IgG4. Two 21OHAb-positive SCA-POI (7%), one 21OHAb-positive AAD (3%), three P450sccAb-positive SCA-POI (15%) and two P450sccAb-positive APS1 (20%) sera were positive for IgG4, in the absence of IgG1. All pre-clinical AAD sera resulted positive for IgG1-21OHAb, but not for IgG4-21OHAb. Conclusions: The autoantibody responses in POI and in AAD are IgG1-dominated, which suggests a predominant Th1 response. Selective IgG4-isotype specificity identified a small subset of patients with Th2-oriented response.

AUTOANTIBODY RESPONSES IN AUTOIMMUNE OVARIAN INSUFFICIENCY AND IN ADDISON DISEASE ARE IGG1-DOMINATED AND SUGGEST A PREDOMINANT, BUT NOT EXCLUSIVE, TH1 TYPE OF RESPONSE

GIORDANO, Roberta;
2010-01-01

Abstract

Objective: Steroid-producing-cell autoantibodies (SCA) directed against 21-hydroxylase (21OHAb), 17α-hydroxylase (17OHAb) and cholesterol side-chain-cleavage enzyme (P450sccAb), characterize autoimmune primary ovarian insufficiency (SCA-POI). The aim of the study was to analyze IgG-subclass specificity of autoantibodies related to adrenal and ovarian autoimmunity. Design: We studied 29 women with SCA-POI, 30 women with autoimmune Addison disease (AAD) without POI and 14 patients with autoimmune polyendocrine syndrome type 1 (APS1). 21OHAb isotypes were also analyzed in 14 subjects with pre-clinical AAD. Samples from 30 healthy women served as control group to determine upper level of normality in the isotype assays. Methods: Immunoradiometric assays with IgG-subclass-specific secondary antibodies. Results: In 21OHAb-positive sera, IgG1 isotype was detected in 90% SCA-POI and non-POI AAD and 67% APS 1 patients. IgG1 isotype was found in 69% 17OHAb-positive SCA-POI and 100% 17OHAb-positive APS1 sera and in 60% P450sccAb-positive SCA-POI and 80% P450sccAb-positive APS1 sera. For 21OHAb, IgG4 isotype was detected in 17% SCA-POI, 7% non-POI AAD and 8% APS1 sera. None of the 17OHAb-positive sera was positive for IgG4. In P450sccAb-positive sera, 15% aPOI and 20% APS1 sera were positive for IgG4. Two 21OHAb-positive SCA-POI (7%), one 21OHAb-positive AAD (3%), three P450sccAb-positive SCA-POI (15%) and two P450sccAb-positive APS1 (20%) sera were positive for IgG4, in the absence of IgG1. All pre-clinical AAD sera resulted positive for IgG1-21OHAb, but not for IgG4-21OHAb. Conclusions: The autoantibody responses in POI and in AAD are IgG1-dominated, which suggests a predominant Th1 response. Selective IgG4-isotype specificity identified a small subset of patients with Th2-oriented response.
2010
163
309
317
A. Brozzetti; S. Marzotti; D. La Torre; M.L. Bacosi; S. Morelli; V. Bini; B. Ambrosi; R. Giordano; R. Perniola; A. De Bellis; C. Betterle; A. Falorni
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/74221
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 23
  • ???jsp.display-item.citation.isi??? 19
social impact