The present study was performed to assess the expression of isoforms 1, 2 and 3 of transforming growth factor (TGF)-beta in skin nodular dermatofibrosis lesions, kidney, bladder and pancreas from a 10-year-old female German shepherd dog (GSD) affected by renal cystadenocarcinoma and nodular dermatofibrosis (RCND) compared with normal GSDs (n = 2). Formalin-fixed, paraffin-embedded tissues obtained from the dog affected by RCND, diagnosed by renal ultrasonography and histopathological examination were analysed by immunohistochemistry using polyclonal antibodies to TGF-beta1, 2 and 3, and evaluated semiquantitatively using an immunoreactivity score. Similar expression of TGF-beta2 and TGF-beta3 was observed in all tissue specimens in both the RCND-affected animal and normal dogs. In contrast, TGF-beta1 immunoreactivity was increased in the derma of the RCND canine. Comparable TGF-beta1 serum levels were found between the diseased and normal animals. The increased local cutaneous production of TGF-beta1 in the RCND dog, compared with the normal animals, suggests that this cytokine may play an important role in the induction of nodular dermatofibrosis associated with renal cystadenocarcinoma.

Expression oftransforming growth factor-beta isoforms in the skin, kidney, pancreas andbladder in a German shepherd dog affected by renal cystadenocarcinoma and nodulardermatofibrosis / Vercelli A; Bellone G; Abate O; Emanuelli G; Cagnasso A.. - In: JOURNAL OF VETERINARY MEDICINE SERIES A. - ISSN 0931-184X. - 50(2003), pp. 506-510.

Expression oftransforming growth factor-beta isoforms in the skin, kidney, pancreas andbladder in a German shepherd dog affected by renal cystadenocarcinoma and nodulardermatofibrosis

BELLONE, Graziella;ABATE, Ornella;EMANUELLI, Giorgio;CAGNASSO, Aurelio
2003

Abstract

The present study was performed to assess the expression of isoforms 1, 2 and 3 of transforming growth factor (TGF)-beta in skin nodular dermatofibrosis lesions, kidney, bladder and pancreas from a 10-year-old female German shepherd dog (GSD) affected by renal cystadenocarcinoma and nodular dermatofibrosis (RCND) compared with normal GSDs (n = 2). Formalin-fixed, paraffin-embedded tissues obtained from the dog affected by RCND, diagnosed by renal ultrasonography and histopathological examination were analysed by immunohistochemistry using polyclonal antibodies to TGF-beta1, 2 and 3, and evaluated semiquantitatively using an immunoreactivity score. Similar expression of TGF-beta2 and TGF-beta3 was observed in all tissue specimens in both the RCND-affected animal and normal dogs. In contrast, TGF-beta1 immunoreactivity was increased in the derma of the RCND canine. Comparable TGF-beta1 serum levels were found between the diseased and normal animals. The increased local cutaneous production of TGF-beta1 in the RCND dog, compared with the normal animals, suggests that this cytokine may play an important role in the induction of nodular dermatofibrosis associated with renal cystadenocarcinoma.
50
506
510
TGF-beta1; dermatofibrosis
Vercelli A; Bellone G; Abate O; Emanuelli G; Cagnasso A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/75121
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