Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F 2 -isoprostanes are widely used as markers of oxidative stress in vitro and in vivo . This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.

An inter-laboratory validation of methods of lipidperoxidation measurement in UVA-treated human plasma samples

BIASI, Fiorella;POLI, Giuseppe;
2010

Abstract

Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F 2 -isoprostanes are widely used as markers of oxidative stress in vitro and in vivo . This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.
44
1203
1215
Oxidative stress; F2-isoprostanes; 4-hydroxynonenal; malondialdehyde
Breusing N; Grune T; Andrisic L; Atalay M; Bartosz G; Biasi F; Borovic S; Bravo L; Casals I; Casillas R; Dinischiotu A; Drzewinska J; Faber H; Fauzi NM; Gajewska A; Gambini J; Gradinaru D; Kokkola T; Lojek A; Luczaj W; Margina D; Mascia C; Mateos R; Meinitzer A; Mitjavila MT; Mrakovcic L; Munteanu MC; Podborska M; Poli G; Sicinska P; Skrzydlewska E; Vina J; Wiswedel I; Zarkovic N; Zelzer S; Spickett CM
File in questo prodotto:
File Dimensione Formato  
FRR 2010 BREUSING.pdf

Accesso aperto con embargo fino al 29/04/2013

Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 402.92 kB
Formato Adobe PDF
402.92 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/75909
Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 52
  • ???jsp.display-item.citation.isi??? 50
social impact