The objective of this study is that of determining the optimal conditions for human semen incubation treated with exogenous Platelet Activating Factor (ePAF) for intra-uterine insemination (IUI). This prospective study was carried out on 32 infertile men and each semen sample was processed with the ISolate Sperm Separation Medium, washed with Sperm Washing Medium (SWM) and resuspended either in SWM alone (control samples), or with ePAF 0.1, 0.5 and 1.0 mu. Each concentration was subsequently incubated and evaluated at 5, 15, 30 and 60 minutes. The motility parameters were evaluated by the Computer Aided Sperm Analysis (C.A.S.A.) system. Curvilinear Velocity (VCL), Straight Line Velocity (VSL), Average Path Velocity (VAP), rapid (RM) and progressive motility (PM) significantly increased compared to the control samples at an ePAF concentration of 0.1 mu (with at least 15 minutes of incubation). The best results were obtained with ePAF concentrations of: 0.1 mu (60 minutes of incubation) and 0.5 mu (30-60 minutes of incubation). In conclusion, results are enhanced when ePAF is added to standard semen preparation for IUI. An ePAF concentration of 0.1 mu, with an incubation time of 15 minutes, can be used for semen samples with normal motility. Whilst, for semen samples with poor motility, the ePAF concentration is best increased to 0.5 mu and/or the incubation time prolonged to 60 minutes.

Exogenous Platelet-Activating Factor improves the motility of human spermatozoa evaluated with C.A.S.A.: optimal concentration and incubation time / Grassi G; Cappello N; Gheorghe MF; Salton L; Di Bisceglie C; Manieri C; Benedetto C.. - In: JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION. - ISSN 0391-4097. - ELETTRONICO. - 33:10(2010), pp. 684-690.

Exogenous Platelet-Activating Factor improves the motility of human spermatozoa evaluated with C.A.S.A.: optimal concentration and incubation time

CAPPELLO, Nazario;SALTON, Loredana;MANIERI, Chiara;BENEDETTO, Chiara
2010

Abstract

The objective of this study is that of determining the optimal conditions for human semen incubation treated with exogenous Platelet Activating Factor (ePAF) for intra-uterine insemination (IUI). This prospective study was carried out on 32 infertile men and each semen sample was processed with the ISolate Sperm Separation Medium, washed with Sperm Washing Medium (SWM) and resuspended either in SWM alone (control samples), or with ePAF 0.1, 0.5 and 1.0 mu. Each concentration was subsequently incubated and evaluated at 5, 15, 30 and 60 minutes. The motility parameters were evaluated by the Computer Aided Sperm Analysis (C.A.S.A.) system. Curvilinear Velocity (VCL), Straight Line Velocity (VSL), Average Path Velocity (VAP), rapid (RM) and progressive motility (PM) significantly increased compared to the control samples at an ePAF concentration of 0.1 mu (with at least 15 minutes of incubation). The best results were obtained with ePAF concentrations of: 0.1 mu (60 minutes of incubation) and 0.5 mu (30-60 minutes of incubation). In conclusion, results are enhanced when ePAF is added to standard semen preparation for IUI. An ePAF concentration of 0.1 mu, with an incubation time of 15 minutes, can be used for semen samples with normal motility. Whilst, for semen samples with poor motility, the ePAF concentration is best increased to 0.5 mu and/or the incubation time prolonged to 60 minutes.
33
10
684
690
http://www.kurtis.it
Grassi G; Cappello N; Gheorghe MF; Salton L; Di Bisceglie C; Manieri C; Benedetto C.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/76153
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