Amphotericin B loaded SLN prepared with the coacervation technique

Stearic acid solid lipid nanoparticles (SLN) were prepared according to the coacervation technique, consisting in fatty acid precipitation of alkaline salt from a solution. SLN can encapsulate Amphotericin B with high efficiency in its monomeric form. SLN markedly increase in vitro drug toxicity towards Saccharomyces cerevisiae (taken as model yeast), expressed as potassium leakage. Yeast susceptibility towards Amphotericin B is increased when the drug is loaded in nanoparticles compared to the free drug, even if inhibition is limited by drug encapsulation within lipid matrix.