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Tissue-specific histone modification and transcription factor binding in alpha globin gene expression.

DE GOBBI, Marco;
2007-01-01
2007
Inglese
WOS:000252001200057
17715390
2-s2.0-39049120185
BLOOD
110
4503
4510
7
http://dx.doi.org/10.1182/blood-2007-06-097964
To address the mechanism by which the human globin genes are activated during erythropoiesis, we have used a tiled microarray to analyze the pattern of transcription factor binding and associated histone modifications across the telomeric region of human chromosome 16 in primary erythroid and nonerythroid cells. This 220-kb region includes the alpha globin genes and 9 widely expressed genes flanking the alpha globin locus. This un-biased, comprehensive analysis of transcription factor binding and histone modifications (acetylation and methylation) described here not only identified all known cis-acting regulatory elements in the human alpha globin cluster but also demonstrated that there are no additional erythroid-specific regulatory elements in the 220-kb region tested. In addition, the pattern of histone modification distinguished promoter elements from potential enhancer elements across this region. Finally, comparison of the human and mouse orthologous regions in a unique mouse model, with both regions coexpressed in the same animal, showed significant differences that may explain how these 2 clusters are regulated differently in vivo.
Acetylation, Animals, Cells; Cultured, Chromosomes; Human; Pair 16, Enhancer Elements; Genetic, Erythroblasts; immunology, Gene Expression Regulation, Globins; genetics, Histones; metabolism, Humans, K562 Cells, Methylation, Mice, Promoter Regions; Genetic, T-Lymphocytes; cytology, Telomere, Transcription Factors; metabolism, Transcription; Genetic; genetics
262
10
M. De Gobbi;E. Anguita;J. Hughes;J. A. Sloane-Stanley;J. A. Sharpe;C. M. Koch;I. Dunham;R. J. Gibbons;W. G. Wood;D. R. Higgs
info:eu-repo/semantics/article
none
03-CONTRIBUTO IN RIVISTA::03A-Articolo su Rivista
03A-Articolo su Rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/89883
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