Abstract We studied the effects of multi-walled carbon nanotubes (MWCNTs) on the electrophysiological properties of cultured mouse chromaffin cells, a model of spontaneously firing cells. The exposure of chromaffin cells to MWCNTs at increasing concentrations (30–263 mg/ml) for 24 h reduced, in a dose-dependent way, both the cell membrane input resistance and the number of spontaneously active cells (from 80–52%). Active cells that survived from the toxic effects of MWCNTs exhibited more positive resting potentials, higher firing frequencies and unaltered voltage-gated Ca2+, Na+ and K+ current amplitudes. MWCNTs slowed down the inactivation kinetics of Ca2+-dependent BK channels. These electrophysiological effects were accompanied by MWCNTs internalization, as confirmed by transmission electron microscopy, indicating that most of the toxic effects derive from a dose-dependent MWCNTs-cell interaction that damages the spontaneous cell activity.

Altered excitability of cultured chromaffin cells following exposure to multi-walled carbon nanotubes.

GAVELLO, DANIELA;VANDAEL, DAVID HENRI FRANCOIS;PREMOSELLI, Federica;MARCANTONI, Andrea;CESANO, Federico;SCARANO, Domenica;FUBINI, Bice;CARBONE, Emilio;FENOGLIO, Ivana;CARABELLI, Valentina
2012

Abstract

Abstract We studied the effects of multi-walled carbon nanotubes (MWCNTs) on the electrophysiological properties of cultured mouse chromaffin cells, a model of spontaneously firing cells. The exposure of chromaffin cells to MWCNTs at increasing concentrations (30–263 mg/ml) for 24 h reduced, in a dose-dependent way, both the cell membrane input resistance and the number of spontaneously active cells (from 80–52%). Active cells that survived from the toxic effects of MWCNTs exhibited more positive resting potentials, higher firing frequencies and unaltered voltage-gated Ca2+, Na+ and K+ current amplitudes. MWCNTs slowed down the inactivation kinetics of Ca2+-dependent BK channels. These electrophysiological effects were accompanied by MWCNTs internalization, as confirmed by transmission electron microscopy, indicating that most of the toxic effects derive from a dose-dependent MWCNTs-cell interaction that damages the spontaneous cell activity.
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Gavello D; Vandael DH; Cesa R; Premoselli F; Marcantoni A; Cesano F; Scarano D; Fubini B; Carbone E; Fenoglio I; Carabelli V.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/92850
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