Sézary syndrome (SS), the leukemic variant of cutaneous T-cell lymphoma (CTCL), has a poor prognosis and infections represent the most frequent cause of death. Polymorphonucleate granulocytes (PMNs) constitute an essential part of the innate immune system: their phagocytic and killing activity against pathogens is mediated by the interactions between Toll-like receptors (TLRs) and the Pathogen-associated molecular patterns (PAMPs). The aim of this study was to investigate PMN functional activity and phenotype in SS patients and their correlation with the onset of infectious complications. This prospective study enrolled 18 consecutive SS patients; PMN functional activity was evaluated by phagocytosis and intracellular killing tests towards Klebsiella pneumoniae. Flow-cytometry was applied to analyze PMN phenotype. PMNs from SS patients displayed a reduced phagocytic activity and intracellular killing against K. pneumoniae at 30 min and 60 min, more pronounced in SS patients with recurrent infections. CD11b and CD66b median fluorescence intensity (MFI) was significantly higher in SS than in healthy subjects, whereas CD62L MFI was decreased. No significant differences in TLR2, 4, 8 and 9 percentage expression or MFI were found. An increased TLR5 percentage expression was documented. The impairment in PMN functional activities in SS could favour the immune-suppression and raise infection risk.

Functional and phenotypical alterations of polymorphonuclear cells in Sézary syndrome patients [*MT. Fierro and AMC Cuffini contributed equally to this work]

FIERRO, Maria Teresa
Co-first
;
CUFFINI, Annamaria
Co-first
;
NOVELLI, Mauro;BANCHE, Giuliana;ALLIZOND, VALERIA;COMESSATTI, Alessandra;BRIZIO, Matteo Giovanni;SCALAS, Daniela;MERLINO, Chiara;QUAGLINO, Pietro;BERNENGO, Maria Grazia
Last
2011-01-01

Abstract

Sézary syndrome (SS), the leukemic variant of cutaneous T-cell lymphoma (CTCL), has a poor prognosis and infections represent the most frequent cause of death. Polymorphonucleate granulocytes (PMNs) constitute an essential part of the innate immune system: their phagocytic and killing activity against pathogens is mediated by the interactions between Toll-like receptors (TLRs) and the Pathogen-associated molecular patterns (PAMPs). The aim of this study was to investigate PMN functional activity and phenotype in SS patients and their correlation with the onset of infectious complications. This prospective study enrolled 18 consecutive SS patients; PMN functional activity was evaluated by phagocytosis and intracellular killing tests towards Klebsiella pneumoniae. Flow-cytometry was applied to analyze PMN phenotype. PMNs from SS patients displayed a reduced phagocytic activity and intracellular killing against K. pneumoniae at 30 min and 60 min, more pronounced in SS patients with recurrent infections. CD11b and CD66b median fluorescence intensity (MFI) was significantly higher in SS than in healthy subjects, whereas CD62L MFI was decreased. No significant differences in TLR2, 4, 8 and 9 percentage expression or MFI were found. An increased TLR5 percentage expression was documented. The impairment in PMN functional activities in SS could favour the immune-suppression and raise infection risk.
2011
21
6
921
929
http://www.jle.com/en/revues/medecine/ejd/e-docs/00/04/70/CC/article.phtml
Sézary syndrome; CTCL; PMN
Fierro, MT*; Cuffini, Annamaria*; Novelli, M; Banche, G; Allizond, V; Comessatti, A; Brizio, M; Scalas, D; Merlino, C; Quaglino, P; Bernengo, Mg....espandi
File in questo prodotto:
File Dimensione Formato  
PMN in SS Eur J Dermatol 2011.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 552.2 kB
Formato Adobe PDF
552.2 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
SS IN PMN Fierro 2011 versione autore con copertina.pdf

Accesso aperto

Descrizione: Articolo principale
Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 1.56 MB
Formato Adobe PDF
1.56 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/95028
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 7
social impact