Curcumin (CU) loaded solid lipid nanoparticles (SLNs) of fatty acids (FA) were prepared with a coacervation technique based on FA precipitation from their sodium salt micelles in the presence of polymeric non-ionic surfactants. Myristic, palmitic, stearic, and behenic acids, and different polymers with various molecular weights and hydrolysis grades were employed as lipid matrixes and stabilisers, respectively. Generally, spherical-shaped nanoparticles with mean diameters below 500nm were obtained, and using only middle-high hydrolysis, grade-polymer SLNs with diameters lower than 300nm were produced. CU encapsulation efficiency was in the range 28–81% and highly influenced by both FA and polymer type. Chitosan hydrochloride was added to FA SLN formulations to produce bioadhesive, positively charged nanoparticles. A CU-chitosan complex formation could be hypothesised by DSC analysis, UV–vis spectra and chitosan surface tension determination. A preliminary study on HCT-116 colon cancer cells was developed to evaluate the influence of CU-loaded FA SLNs on cell viability.

Formulation of curcumin-loaded solid lipid nanoparticlesproduced by fatty acids coacervation technique

CHIRIO, Daniela;GALLARATE, Marina;PEIRA, Elena;BATTAGLIA, Luigi Sebastiano;SERPE, Loredana;TROTTA, Michele
2011

Abstract

Curcumin (CU) loaded solid lipid nanoparticles (SLNs) of fatty acids (FA) were prepared with a coacervation technique based on FA precipitation from their sodium salt micelles in the presence of polymeric non-ionic surfactants. Myristic, palmitic, stearic, and behenic acids, and different polymers with various molecular weights and hydrolysis grades were employed as lipid matrixes and stabilisers, respectively. Generally, spherical-shaped nanoparticles with mean diameters below 500nm were obtained, and using only middle-high hydrolysis, grade-polymer SLNs with diameters lower than 300nm were produced. CU encapsulation efficiency was in the range 28–81% and highly influenced by both FA and polymer type. Chitosan hydrochloride was added to FA SLN formulations to produce bioadhesive, positively charged nanoparticles. A CU-chitosan complex formation could be hypothesised by DSC analysis, UV–vis spectra and chitosan surface tension determination. A preliminary study on HCT-116 colon cancer cells was developed to evaluate the influence of CU-loaded FA SLNs on cell viability.
28
537
548
curcumin; SLN; fatty acids; coacervation
DANIELA CHIRIO; MARINA GALLARATE; ELENA PEIRA; LUIGI BATTAGLIA ; LOREDANA SERPE; MICHELE TROTTA
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/95359
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