The present research investigated in vitro seed germination and seedling multiplication methods to preserve and introduce native valuable Campanula species into the floriculture markets. Populations of Campanula barbata L., Campanula latifolia L., Campanula rapunculoides L., Campanula spicata L., and Campanula trachelium L. from various Northern Italy sites were considered. Seeds were sown on a half-strength Murashige & Skoog (MS) basal medium with 1-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and kinetin, added or withheld. Final Germination Percentage (FGP), Mean Germination Time (MTG), and Time of 50% Germination (T50) indices were estimated. These indices were poorly affected by the presence of phytohormones. MGT was influenced only in C. latifolia, while none differences were detected concerning the T50. C. rapunculoides demonstrated the highest FGP after two weeks in both the presence (53%) and absence (56%) of plant growth regulators, C. barbata the lowest (nearly o%). Among the other species, no statistical differences were detected, except for C. spicata that was negatively influenced by the presence of growth regulators. After four months, the seedlings were transferred to full-strength MS basal medium with NAA and BA, added or withheld. The highest shooting and rooting rates were observed on substrates with plant growth regulators, in all the Campanula species.
Studio sulla germinazione e propagazione in vitro nel genere Campanula
SEGLIE, LUDOVICA;SCARIOT, VALENTINA;DEVECCHI, Marco
2011-01-01
Abstract
The present research investigated in vitro seed germination and seedling multiplication methods to preserve and introduce native valuable Campanula species into the floriculture markets. Populations of Campanula barbata L., Campanula latifolia L., Campanula rapunculoides L., Campanula spicata L., and Campanula trachelium L. from various Northern Italy sites were considered. Seeds were sown on a half-strength Murashige & Skoog (MS) basal medium with 1-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and kinetin, added or withheld. Final Germination Percentage (FGP), Mean Germination Time (MTG), and Time of 50% Germination (T50) indices were estimated. These indices were poorly affected by the presence of phytohormones. MGT was influenced only in C. latifolia, while none differences were detected concerning the T50. C. rapunculoides demonstrated the highest FGP after two weeks in both the presence (53%) and absence (56%) of plant growth regulators, C. barbata the lowest (nearly o%). Among the other species, no statistical differences were detected, except for C. spicata that was negatively influenced by the presence of growth regulators. After four months, the seedlings were transferred to full-strength MS basal medium with NAA and BA, added or withheld. The highest shooting and rooting rates were observed on substrates with plant growth regulators, in all the Campanula species.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
